|
|  | |
| | ⇒ Color reactions of carbohydrates
⇒ Determination of amylose
⇒ Determination of fructose and inulin
⇒ Determination of glucose by glucose oxidase method
⇒ Determination of reducing sugars by Nelson-Somogyi method
⇒ Determination of total carbohydrates by anthrone method
⇒ Estimation of cellulose
⇒ Estimation of crude fiber
⇒ Estimation of hemicelluloses
⇒ Estimation of pectic substances by:
⇒ Colorimetric method
⇒ Gravimetric method
⇒ Estimation of reducing sugar by dinitrosalicylic acid method
⇒ Estimation of starch by anthrone reagent
⇒ Phenol suplhuric acid method for total carbohydrate | |
|
| |
| | |
|  | |
| | ⇒ Determination of iodine value of oil
⇒ Determination of peroxide value
⇒ Estimation of free fatty acids
⇒ Estimation of oil in oilseeds
⇒ Estimation of volatile acids
⇒ Identification of quantification of fatty acids
⇒ Saponification value | |
|
| |
| | |
|  | |
| | ⇒ Ammonium sulphate fractionation of proteins
⇒ Determination of net protein utilization, digestibility and biological value
⇒ Electrophoresis of proteins in starch gels
⇒ Enzyme-linked immunosorbant assay (ELISA)
⇒ Estimation of lysine
⇒ Estimation of methionine in food grains
⇒ Estimation of proline
⇒ Estimation of total free amino acids
⇒ Estimation of tryptophan
⇒ Fluorography of polyacrylamide gels
⇒ Identification of proteins
⇒ Immunodiffusion in agrose gels
⇒ In vitro translation assay
⇒ Isoelectric focusing
⇒ Mass Screening test for high-lysine grains
⇒ Nitrogen analysis by micro-Kjeldahl method
⇒ Polyacrylamide disc gel electrophoresis
⇒ Polyacrylamide-sodium dodecyl sulphate slab gel electrophoresis (SDS-PAGE) of proteins
⇒ Preparation S-30 extract for protein synthesis in vitro
⇒ Production of antiserum
⇒ Protein (Western) blotting
⇒ Protein digestibility in vitro
⇒ Protein efficiency and net protein ratio
⇒ Protein estimation – Bradford method
⇒ Protein estimation by Lowry’s method
⇒ Quantification of proteins in polyacrylamide gels
⇒ Rocket immunoelectrophoresis
⇒ Sample preparation for amino acids estimation
⇒ Silver staining of proteins | |
|
| |
| | |
|  | |
| | ⇒ Acetyl cholinesterase
⇒ Aminotransferases
i) Asparatate amino transferase
ii) Alanine aminotransferase
⇒ Amylases
⇒ Ascorbic acid oxidase
⇒ Asparagine synthetase
⇒ Catalase
⇒ Cellulases (C1 and Cx)
i) Viscometric method
ii) Colorimetric method
iii) Combined assay
⇒ Glutamate dehydrogenase
⇒ Glutamate synthase
⇒ Glutamine synthetase
⇒ Indole acetic acid oxidase
⇒ Isoenzyme analysis
i) Esterase
ii) Polyphenol oxidase (PPO)
iii) Peroxidase
iv) Acid phosphatase
v) Glutamine synthetase
vi) Phosphoenol pyruvate carboxylase (PEP Case)
vii) Glutamate dehydrogenase
viii) Indolylacetic acid oxidase
ix) Malate dehydrogenase
x) Sucrose synthase
xi) Aspartate aminotransferase
xii) Glutamate synthase
⇒ Lipase
⇒ Malate dehydrogenase
⇒ Nitrate reductase
⇒ Nitrite reductase
⇒ Papain
⇒ Pectin methyl esterase
⇒ Peroxidase
⇒ Phenylalanine ammonia lyase
⇒ Phosphatases
i) Acid phosphatase
ii) Assay using glycerophosphate
⇒ Phosphoenol pyruvate carboxylase
⇒ Polygalacturonase activity
⇒ Polyphenol oxidase
⇒ Ribulose biphosphate carboxylase
⇒ Sucrose synthase | |
|
| |
| | |
|  | |
| | ⇒ Cultivation of lambda (l) phage
⇒ DNA electrophoresis in agarose gel
⇒ Estimation of DNA
⇒ Estimation of RNA
⇒ Extraction of lambda (l) DNA
⇒ Extraction of plant DNA
⇒ Extraction of plant total DNA (Rapid method)
⇒ Isolation of deoxyribonucleic acid (DNA)
⇒ Isolation of messenger RNA by affinity chromatography
⇒ Isolation of plasmids
⇒ Isolation of ribonucleic acid
⇒ Rapid isolation of plasmid DNA (Miniscreen)
⇒ Recovery of DNA fragments from agarose gels
⇒ Restriction of DNA
⇒ Southern blotting | |
|
| |
| | |
|  | |
| | ⇒ Ascorbic acid
i) Volumetric method
ii) Colorimetric analysis
⇒ Niacin
⇒ Riboflavin
⇒ Thiamine | |
|
| |
|
| |
|  | |
| | ⇒ Carotenes
⇒ Chlorophylls
⇒ Curcumin
⇒ Lycopene | |
|
| |
| | |
|  | |
| | ⇒ Capsaicin
i) Colorimetric Method
ii) Spectrophotometric Method
⇒ Chlorogenic acid
⇒ Lignins
⇒ Phenols
⇒ Tannins
i) Folin-Denis Method
ii) Vanillin Hydrochloride Method | |
|
| |
| | |
|  | |
| | ⇒ Cyanogens
⇒ Gossypol
⇒ Phytic acid
⇒ Trypsin inhibitor | |
|
| |
| | |
|  | |
| | ⇒ Ethylene
⇒ Indoleacetic acid | |
|
| |
| | |
|  | |
| | ⇒ Allantoin and Allantoic acid
⇒ Hydrogen evolution
⇒ Leghaemoglobin
⇒ Nitrogenase | |
|
| |
| | |
|  | |
| | ⇒ Analysis of aflatoxins
⇒ Column chromatography
⇒ Paper Chromatography
⇒ Thin layer chromatography | |
|
| |
| | |
|  | |
| | ⇒ Fractionation of cellular components
⇒ Isolation of chloroplasts
⇒ Isolation of mitochondria |
